Abcam, ab215366, Anti-TXNIP antibody [EPR14774] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TXNIP antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14774,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Expression levels of the target protein vary with sample type and some optimisation may be required. For western blotting, more concentrated lysates may be required when using tissues samples.Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.

Product details:
ab215366 is the carrier-free version of
ab188865
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TXNIP also known as thioredoxin-interacting protein or VDUP1 is an inhibitory protein that binds to and regulates the activity of thioredoxin. It has a molecular mass of around 46 kDa. TXNIP is expressed in various tissues including heart liver and skeletal muscle. It acts to control intracellular redox states by modulating thioredoxin’s antioxidant functions. TXNIP expression can vary according to oxidative stress and cellular metabolic conditions.
Biological function summary
TXNIP regulates cellular processes such as glucose metabolism stress response and apoptosis. It does not form a complex but functions by negatively regulating the activity of thioredoxin. This protein can lead to increased reactive oxygen species (ROS) when it inhibits thioredoxin influencing cellular redox balance. TXNIP therefore plays a regulatory role in metabolic pathways and cellular stress responses by modulating thioredoxin activity and ROS levels.
Pathways
TXNIP is a significant component of the oxidative stress and glucose metabolism pathways. In the oxidative stress pathway TXNIP interacts with thioredoxin influencing ROS detoxification and redox-sensitive signaling. In glucose metabolism TXNIP can affect insulin signaling by interacting with glucose transporter-1 (GLUT1). These interactions highlight TXNIP's regulatory role in cellular metabolism and stress-related signaling making it a node of convergence with proteins like thioredoxin and GLUT1.
TXNIP levels have been implicated in conditions such as type 2 diabetes and cardiovascular diseases. Elevated TXNIP expression has been observed in insulin resistance where it affects glucose uptake by modifying GLUT1 activity and links to thioredoxin in cardiovascular tissues resulting in oxidative stress damage. These connections position TXNIP as a potential therapeutic target for managing metabolic disorders and cardiovascular complications.