Abcam, ab215367, Anti-STAT5a + STAT5b antibody [EPR16671-40] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal STAT5a antibody. Carrier free. Suitable for WB, IP, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16671-40,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, IP, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab215367 is the carrier-free version of
ab194898
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
STAT5a and STAT5b known jointly as members of the Signal Transducers and Activators of Transcription family play critical roles as transcription factors. They have molecular masses of approximately 94 kDa and 92 kDa respectively. These proteins share significant sequence similarity and are expressed in a wide range of tissues including hematopoietic cells liver and mammary glands. STAT5 proteins become activated in response to cytokines and growth factors where they dimerize and translocate to the cell nucleus to regulate gene expression.
Biological function summary
They are involved in mediating cytokine signaling by forming homo- or heterodimers upon phosphorylation by Janus kinases (JAKs). In the cytoplasm the formation of these dimers allows STAT5 to interact with DNA in the nucleus leading to the transcription of various genes important for cell proliferation differentiation and apoptosis. STAT5 has a significant role in cell cycle control and immune responses. The dimerization process involves complex formation and is an important part of its intracellular signaling cascade.
Pathways
STAT5a and STAT5b operate prominently within the JAK-STAT pathway. They are activated by proteins such as IL-2 IL-3 and Growth Hormone leading to transcriptional activity that affects growth and survival across various cell types. Besides the JAK-STAT pathway STAT5 interacts closely with the PI3K/AKT pathway thereby linking to cell survival signals. The cross-talk between these pathways enables fine-tuning of cellular responses to external stimuli and integrates signals from diverse receptors and ligands.
STAT5 deregulated activity associates with various cancers such as leukemia and breast cancer due to its role in promoting excessive cell proliferation and survival. Abnormal activity in STAT5 can also contribute to disorders like autoimmune diseases due to its impact on immune cell regulation. In diseases like these STAT5 interacts notably with proteins such as BCL-2 and MYC which are critical modulators of cell death and growth pathways. Understanding the involvement of STAT5 in disease mechanisms provides opportunities for developing targeted therapeutics.