Abcam, ab215396, Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker

Size: 100µg
Mouse Monoclonal Human Nuclear Antigen antibody. Nucleus marker. Suitable for IHC-P, ICC/IF, Flow Cyt and reacts with Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Nuclei of HL60 cells.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:NM106,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Rat, Human,
Applications:ICC/IF, IHC-P, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-ab215396 was purified from Bioreactor Concentrate by Protein A/G., Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nuclear Antigen often also referred to as the nuclear antigen (NA) or nuclear matrix protein performs important mechanical roles within the cell nucleus. The protein has an approximate mass of around 120 kDa and mostly localizes within the nuclear compartment of cells. Researchers have observed that Nuclear Antigen binds tightly to chromatin and plays a part in maintaining nuclear integrity and organization. This target shows expression in proliferative tissues and is linked with cell cycle regulation.
Biological function summary
Nuclear Antigen provides critical involvement in DNA replication and repair processes. It serves as a scaffold within the nucleus facilitating interactions between DNA and various nuclear enzymes. Nuclear Antigen acts as part of the nuclear matrix complex supporting cellular processes that maintain genomic stability. By modulating the structure of chromatin Nuclear Antigen influences gene expression and cell proliferation making it a noteworthy player in regulating cellular growth.
Pathways
The Nuclear Antigen influences cellular processes like cell cycle control and apoptosis. It interacts closely with pathways including the DNA damage response pathway and cell cycle checkpoints. Within these pathways its interaction with proteins such as p53 and BRCA1 is of particular importance. These interactions assist in the coordination of repair mechanisms during DNA damage events ensuring proper cell cycle progression and preventing genomic instability.
Studies link Nuclear Antigen to specific types of cancer and autoimmune diseases. Its association with cancer comes from its role in cell cycle regulation as its altered expression is seen in several tumor types. Conversely in autoimmune disorders Elevated nuclear antigen expression levels can elicit autoimmune reactions suggesting their significant role. Here the protein interacts with disease-related molecules such as oncogenes in cancer or autoantibodies in autoimmune conditions highlighting its contribution to disease mechanisms.