Abcam, ab215823, Anti-AUH antibody [EPR11087(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal AUH antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR11087(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab215823 is the carrier-free version of
ab155980
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AUH also known as AU-specific RNA binding protein/enoyl-CoA hydratase is a bifunctional protein with a molecular mass of approximately 39 kDa. It plays an important role in RNA binding and hydratase activities. AUH is expressed in many tissues including liver brain and heart providing its multiple functional capacities within cells. It binds to AU-rich elements present in mRNA which has significant influence on mRNA stability and turnover. The protein also acts as an enoyl-CoA hydratase involved in the leucine catabolic process.
Biological function summary
The AUH protein is involved in regulating the stability of mRNA by binding to AU-rich elements. This activity is critical for controlling genes expression as it modulates the decay rate of various mRNAs involved in cellular responses. While AUH functions largely independently it may interact with other proteins transiently to exert its RNA binding activity although it is not a part of a stable protein complex. Its hydratase function contributes to the breaking down of leucine an essential amino acid further highlighting its multifaceted biological roles.
Pathways
AUH is interwoven with essential metabolic and gene expression pathways. The protein aligns heavily within the leucine catabolism pathway contributing to the conversion of leucine to acetoacetate and acetyl-CoA which are key intermediates in energy production. AUH's involvement in mRNA decay processes connects it to pathways regulating apoptosis and proliferation where precise control of mRNA turnover by AUH and its related proteins like HuR is essential for cellular outcome.
AUH is linked to a rare genetic disorder known as 3-Methylglutaconic aciduria type 1 (MGA-1) characterized by metabolic anomalies manifesting due to defective leucine catabolism. Mutations in the AUH gene disrupt normal protein function leading to accumulation of metabolic intermediates. Furthermore AUH's role in mRNA regulation implies a connection to cancer where altered expression or mutation might contribute to disruptions in gene expression control. Associated proteins in these contexts include components of the mRNA decay pathway and enzymes involved in amino acid metabolism.