Abcam, ab215824, Anti-TAF3 antibody [EPR15598] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal TAF3 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15598,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab215824 is the carrier-free version of
ab188332
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TAF3 also known as TATA-box binding protein associated factor 3 is an important component of the transcription factor IID (TFIID) complex. This protein weighs approximately 98 kDa. It is expressed in various tissues with high expression in the brain and testis. TAF3 interacts directly with the core promoter elements in DNA enabling the accurate initiation of transcription by RNA polymerase II. This interaction is stabilized by the YEATS domain within TAF3 which binds acetylated histones thereby playing a role in the regulation of transcription elongation.
Biological function summary
TAF3 plays an essential role in cellular processes involving transcriptional regulation and chromatin structure modification. TAF3 is part of the larger TFIID complex which is necessary for the initiation of transcription by RNA polymerase II. It acts by binding specific DNA elements thereby facilitating the recruitment of additional transcription machinery. The presence of the YEATS domain enables TAF3 to bind to modified histones which can alter chromatin architecture to promote gene expression.
Pathways
TAF3 functions within the transcription initiation pathway involving RNA polymerase II. TFIID which includes TAF3 positions itself at the core promoter of genes making it an integral part of RNA polymerase II-dependent transcription regulation. Its interactions are significant in the Wnt signaling pathway in which it facilitates the expression of target genes. In this context TAF3 works alongside proteins like TATA-binding protein (TBP) and other TAFs (TBP-associated factors) to ensure proper transcriptional activation.
TAF3 holds relevance in cancer and neurological disorders. Its role in the transcriptional regulation of genes critical for cell proliferation and differentiation can have implications for tumorigenesis. Aberrant expression of TAF3 is linked to certain forms of cancer where deregulation of transcriptional initiation pathways can lead to uncontrolled cell growth. Furthermore TAF3's involvement in transcriptional modulation makes it a candidate in studying neurodegenerative diseases potentially through interactions with proteins that influence neural gene expression and stability such as histone modifications.