Abcam, ab215838, Anti-pan Cytokeratin antibody [PAN-CK (Cocktail)]

Size: 100µg
Mouse Monoclonal Cytokeratin 2P antibody. Suitable for ICC, Flow Cyt, IHC-P and reacts with Human samples. Cited in 38 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KRT76.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:PAN-CK (Cocktail),
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, Flow Cyt, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human KRT76.Q01546,
Specificity:This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) Cytokeratins, with 67kDa (Cytokeratin1); 64kDa (Cytokeratin3); 59kDa (Cytokeratin4); 58kDa (Cytokeratin5); 56kDa (Cytokeratin6); 55kDa (Cytokeratin7); 52kDa (Cytokeratin8); 56.5kDa (Cytokeratin10); 53kDa (Cytokeratin13); 50kDa (Cytokeratin14); 50kDa (Cytokeratin15); 48kDa (Cytokeratin16); 46kDa (Cytokeratin17); 45kDa (Cytokeratin18) and 40kDa (Cytokeratin19).

Product details:
Isotype is mouse IgGs, kappa.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Purification notes-ab215838 is purified from Bioreactor Concentrate by Protein A/G., Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.
Biological function summary
Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.
Pathways
Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).
Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.