Abcam, ab216572, Anti-PKA alpha + beta (catalytic subunits) antibody

Size: 100µL
Rabbit Polyclonal PKA beta (catalytic subunit) antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Cow PRKACB aa 1-100 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Cow PRKACB aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P05131

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein Kinase A (PKA) alpha and beta catalytic subunits are essential components in cellular signaling. PKA also known as cAMP-dependent protein kinase has its catalytic subunits encoded by PRKACA (alpha) and PRKACB (beta) genes. Alpha subunit has a mass of approximately 40-45 kDa. These proteins are expressed in various tissues including the brain heart and skeletal muscle. They play an important role in phosphorylating target proteins in response to increased cellular levels of cyclic adenosine monophosphate (cAMP).
Biological function summary
PKA alpha and beta catalytic subunits participate in many cellular processes. They are part of a larger complex with regulatory subunits that inhibit their activity. The complex remains inactive until cAMP binds to the regulatory subunits causing a conformational change that activates the catalytic subunits. Once activated PKA subunits phosphorylate various substrates to modulate functions such as metabolism transcription and memory.
Pathways
PKA catalytic subunits serve as key mediators in the cAMP signaling pathway. This pathway influences cellular responses to hormones like adrenaline and glucagon. As such PKA often interacts with proteins such as CREB (cAMP response element-binding protein) to regulate gene expression. PKA also has a role in the regulation of glycogen sugar and lipid metabolism working in concert with proteins like phosphorylase kinase.
Aberrant regulation of PKA catalytic subunits can lead to various conditions. Overactive or dysregulated PKA is associated with Cushing's syndrome often due to mutations in PRKACA. Additionally these catalytic subunits are implicated in certain cancers through alterations in the cAMP signaling pathway. The interaction between PKA and other proteins such as RII-beta (a regulatory subunit) is critical in understanding the molecular basis of these diseases.