Abcam, ab21685, Anti-GRP78 BiP antibody

Size: 100µg
Anti-GRP78 BiP antibody (ab21685) is a rabbit polyclonal antibody detecting GRP78 BiP in Western Blot, IHC-P, ICC/IF . Suitable for Chinese hamster, Human, Mouse, Rat . - Over 640 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Chinese hamster,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Replenishment batches of our polyclonal antibody, ab21685 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab108613.

Product details:
Anti-GRP78 BiP antibody (ab21685) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human, mouse, recombinant fragment samples.
Anti-GRP78 BiP antibody (ab21685) has been cited over 646 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-GRP78 BiP antibody (ab21685) has high sensitivity and specificity.
Anti-GRP78 BiP antibody (ab21685) has 71 independent reviews from customers.
Anti-GRP78 BiP antibody (HSPA5 antibody) (ab21685) specifically detects GRP78 BiP (UniProt ID: P20029; Molecular weight: 70kDa) and is sold in 100 µg selling sizes.
GRP78 (Glucose-Regulated Protein 78), also known as BiP(Binding Immunoglobulin Protein) or heat shock 70 kDa protein 5 (HSPA5), is a crucial endoplasmic reticulum (ER) chaperone protein that is often overexpressed in cancer cells. It helps cancer cells survive under stressful conditions by regulating the ER stress response and preventing apoptosis. Additionally, GRP78 helps maintain the tumor microenviroment and support cancer stem cells properties, promoting tumor growth and metastasis.
Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GRP78 also known as BiP or HSPA5 is a protein that plays an important role in protein folding and assembly within the endoplasmic reticulum (ER). It weighs approximately 78 kDa hence the name GRP78. This chaperone protein binds to hydrophobic regions of nascent polypeptides to prevent aggregation and misfolding. GRP78 is expressed in high levels in the ER of cells where it monitors cellular stress and aids in maintaining ER homeostasis.
Biological function summary
GRP78/BiP is important in the unfolded protein response (UPR) a cellular stress response related to the ER. It is part of a complex that manages protein load inside the ER by regulating the fold of nascent proteins and interacting with other ER stress sensors like IRE1 PERK and ATF6. This function is essential for maintaining proper protein conformation in the ER especially during physiological stress ensuring that only correctly folded proteins proceed to the Golgi apparatus.
Pathways
GRP78/BiP significantly affects the UPR and apoptosis pathways. By controlling protein folding and quality in the ER GRP78 helps prevent cell death under stress conditions. It works closely with other proteins like ATF6 which activates stress response genes. In normal conditions GRP78 keeps stress transducers inactivated but during stress it dissociates allowing UPR signaling to occur.
GRP78/BiP has been implicated in cancer and neurodegenerative diseases. Overexpression of GRP78 is associated with tumor proliferation and poor prognosis in various cancers as cancer cells heavily rely on its protein-folding capacity to survive. Additionally in neurodegenerative diseases misfolded proteins can accumulate leading GRP78 to attempt counteracting these toxic aggregations highlighting its connection with proteins like tau and amyloid-beta in diseases such as Alzheimer's.