Abcam, ab217065, Anti-ARPC4 antibody

Size: 100µg
Rabbit Polyclonal ARPC4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ARPC4 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ARPC4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.P59998

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab217065 was affinity purified using an epitope specific to ARPC4 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ARPC4 protein also known as Actin-Related Protein 2/3 Complex Subunit 4 plays a significant role in the actin nucleation process. It is a component of the ARP2/3 complex which is essential for the formation of branched actin filament networks. ARPC4 has a molecular weight of approximately 20 kDa. It is ubiquitously expressed across various tissues indicating its fundamental role in cellular mechanisms.
Biological function summary
ARPC4 contributes to the dynamic remodeling of the actin cytoskeleton. It is an integral part of the ARP2/3 complex a multi-protein structure that facilitates actin filament branching promoting cell movement and shape changes. This complex is critical for processes like cell migration phagocytosis and intracellular trafficking. By aiding in these activities ARPC4 influences cellular responses to environmental signals and stress.
Pathways
Proteins related to cellular movement and structure rely significantly on actin network regulation. ARPC4 through the ARP2/3 complex participates in the regulation of the WAVE regulatory complex (WRC) and is involved in the actin-related pathways that include Rac1 and N-WASP. These pathways are key to transmitting signals that control cytoskeletal reorganization and influence cell polarity movement and division.
Aberrations in the actin filament network including those involving ARPC4 can contribute to pathological conditions. Research suggests that disruptions in ARPC4 function are linked to cancer metastasis due to its role in cell motility. Additionally ARPC4 has connections with neurological disorders through its interaction with proteins like N-WASP and Rac1 which are known to influence neural cell signaling and structure. Understanding the role of ARPC4 in these diseases may offer potential therapeutic targets.