Abcam, ab217622, Anti-CENPA antibody

Size: 100µL
Rabbit Polyclonal CENPA antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CENPA aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CENPA aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P49450

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Proclin 300Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CENPA also known as Centromere Protein A is an essential component of the centromere specifically during cell division. It represents a histone H3 variant with an approximate mass of 17 kDa. This protein is expressed at the centromere regions of chromosomes where it substitutes conventional histone H3. CENPA facilitates the assembly of the kinetochore an important structure for chromosome segregation. Scientists also refer to this protein as CENP-A often in conjunction with studies involving anti-centromere antibodies and centromere staining.
Biological function summary
CENPA plays a central role in accurate chromosome segregation during mitosis and meiosis. It incorporates into nucleosomes at the centromere replacing standard histone H3 to promote kinetochore formation. CENPA is part of a larger protein complex known as the centromere. This complex propelled by CENPA drives proper attachment of chromosomes to spindle microtubules preventing aneuploidy.
Pathways
The kinetochore pathway and the chromosome segregation pathway both involve CENPA significantly. It functions in tandem with other centromeric proteins such as CENP-B and CENP-C which work collaboratively to sustain centromere integrity. CENPA interacts with these proteins to orchestrate correct alignment and separation of chromosomes integrating it into the foundational architecture of genetic stability.
CENPA has association with several chromosomal instability syndromes. Misregulation or mutation of this protein may contribute to cancer progression where improper chromosome segregation is an important factor. It also connects to other proteins like CENP-H implicated in malignancies due to errors in chromosome transmission. Additionally autoimmune diseases often involve centromere antibodies highlighting the connection of CENPA to disorders such as systemic sclerosis.