Abcam, ab217678, Anti-Rad21 antibody [EPR22506-15] - ChIP Grade

Size: 100µL / 1mL
Anti-Rad21 antibody [EPR22506-15] - ChIP Grade (ab217678) is a rabbit monoclonal antibody detecting Rad21 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, ChIP . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22506-15,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), ChIP, WB, IHC-P, ICC/IF, ChIC/CUT&RUN-seq, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-Rad21 antibody [EPR22506-15] - ChIP Grade (ab217678) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Human, Mouse, Rat samples.
What is the molecular weight of Rad21?
Anti-Rad21 [EPR22506-15] - ChIP Grade (ab217678) specifically detects a band for Rad21 (UniProt: O60216) at a molecular weight of 72kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
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ab254483
, APC -
ab305774
, HRP -
ab305775
, Alkaline Phosphatase -
ab308643
, Alexa Fluor® 488 -
ab309618
, Alexa Fluor® 647 -
ab309984
, Alexa Fluor® 594 -
ab310345
, Alexa Fluor® 555 -
ab311867
, Alexa Fluor® 568 -
ab312336
, Alexa Fluor® 750 -
ab321393
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rad21 also known as double-strand-break repair protein rad21 homolog serves as a component of the cohesin complex. This protein is approximately 123 kDa in size. Rad21 is expressed in a wide range of tissues and cell types particularly in those with high proliferative activity. The protein plays an essential mechanical role by facilitating the cohesion of sister chromatids during cell division ensuring successful chromosome segregation.
Biological function summary
Rad21 is essential in processes beyond chromatid cohesion. It forms part of the cohesin complex which also includes proteins like SMC1 SMC3 and SCC3/SA1 or SA2. This complex is central to maintaining genome stability by participating in DNA repair and regulating gene expression. Through these roles Rad21 contributes to cellular processes such as cell cycle progression and embryonic development.
Pathways
Rad21 integrates into the DNA damage response and cell cycle regulation pathways. The cohesin complex with Rad21 as an important factor acts alongside other proteins such as ATM and ATR in the DNA damage response ensuring the repair of double-strand breaks. Within the cell cycle Rad21 helps coordinate the timing and accuracy of cell division working with cyclin-dependent kinases.
Rad21 has been linked to cancer and Cornelia de Lange syndrome. Mutations or dysregulation of Rad21 can result in tumorigenesis due to errors in chromosome segregation and compromised DNA repair. In Cornelia de Lange syndrome cohesin-related proteins like NIPBL and SMC1A also play a role indicating a disruption in the cohesin pathway's function. Understanding Rad21's involvement helps in developing targeted therapies for these conditions.