Abcam, ab218017, Anti-ERK1 + ERK2 antibody [EPR17526] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ERK1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse, Recombinant full length protein - Human samples. Cited in 6 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17526,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab218017 is the carrier-free version of
ab184699
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ERK1 and ERK2 also known as MAPK3 and MAPK1 respectively are protein kinases involved in the mitogen-activated protein kinase (MAPK) signaling pathway. They share high sequence identity and exhibit similar functions. ERK1 has a molecular weight of approximately 44 kDa while ERK2 weighs around 42 kDa. Both are expressed ubiquitously in various tissues playing roles in diverse cellular processes. These proteins often detected through ERK1/2 western blot analyses present similar ERK protein size and ERK molecular weight characteristics relevant during protein expression studies.
Biological function summary
ERK1 and ERK2 play key roles in cell proliferation differentiation and survival. They form part of a cascade that includes upstream activators such as MEK1/2 and downstream targets including transcription factors. As components of the MAPK signaling complex ERK1/2 regulate gene expression through phosphorylation events impacting cellular responses to various stimuli. Their activation often hinges on growth factors cytokines and stress signals facilitating cellular adaptation to environmental changes.
Pathways
Regarding pathways ERK1/2 sit within the MAPK/ERK pathway and are significant in the Ras/Raf/MEK/ERK cascade one of the foremost signaling mechanisms in cells. They interact with several proteins including Ras and Raf which modulate their activation. This pathway is important for transmitting signals from the cell surface to the DNA in the cell nucleus impacting gene regulation and cell fate decisions. ERK1/2 proteins therefore serve as critical nodes linking extracellular signals to cellular responses ensuring balanced cell function.
ERK1/2 play significant roles in cancer and neurodegenerative diseases. Their overactivation is often linked to oncogenic processes contributing to cell proliferation in cancers. Mutations or dysregulation within the MAPK/ERK pathway including ERK1/2 frequently associate with tumorigenesis. Additionally in neurodegenerative disorders like Alzheimer's disease altered ERK1/2 activity may impact neuronal survival and function often through interaction with amyloid-beta and tau proteins further illustrating their importance in disease pathophysiology.