Abcam, ab218675, Recombinant human DEFB-4 protein (Active)

Size: 5µg
Recombinant human DEFB-4 protein (Active) is a Human Full Length protein, in the 23 to 72 aa range, expressed in Escherichia coli, with >98%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.
Key facts
Purity:>98% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:HPLC, FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1 -100.0 ng/ml.,
Accession:Q8WTQ1,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in water or aqueous buffer,
Storage buffer:pH: 7.4Constituents: 99% Phosphate Buffer, 0.75% Sodium chloride

Product details:
Protein previously labeled as beta 4 Defensin.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate long-term storage conditions--20°C, Storage information-Store under desiccating conditions

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DEFB-4 also known as beta-defensin 4 or human beta-defensin 2 (hBD-2) is a small antimicrobial peptide with a mass of about 4.5 kDa. This peptide belongs to the defensin family which plays a role in the immune response. DEFB-4 is expressed in epithelial cells including those in the skin respiratory tract and urogenital tract. It is induced by microbial presence and inflammatory cytokines such as IL-1 and TNF-alpha.
Biological function summary
This peptide exhibits antimicrobial activity against bacteria fungi and viruses. DEFB-4 disrupts microbial membranes leading to microbe death. It does not form part of a larger protein complex but it plays an important role in immune defense by recruiting and activating immune cells. It also exhibits chemotactic properties suggesting an involvement in modulating immune responses and interactions with other innate immune system components.
Pathways
DEFB-4 is involved in the innate immune pathway that mediates early responses to infections. Its interaction with the Toll-like receptor (TLR) pathways further enhances cytokine production. DEFB-4 acts alongside other beta-defensins like DEFB-1 contributing to a broader antimicrobial defensive network. Activation of these pathways results in coordinated immune responses that are critical during the early stages of microbial invasion.
The dysregulation of DEFB-4 expression associates with conditions such as psoriasis and cystic fibrosis. In psoriasis increased expression of DEFB-4 correlates with persistent inflammation and immune activity in the skin. In cystic fibrosis impaired DEFB-4 function can lead to an increased risk of chronic lung infections. The protein's relationship to IL-1 and TNF-alpha plays a role in these inflammatory conditions making DEFB-4 a target of interest for therapeutic research.