Abcam, ab218819, Recombinant Human Alpha-synuclein protein aggregate (Active)

Size: 100µg
Recombinant Human Alpha-synuclein protein aggregate (Active) is a Human Full Length SNCA protein, in the 1 to 140 aa range with >95% purity, <= 20 EU/mL endotoxin level and suitable for western blot, Functional studies and SDS-PAGE. The predicted molecular weight of ab218819 recombinant protein is 14.5 kDa. - Suitable for Thioflavin T Fluorescence assay - Save time and ensure accurate results - use recombinant Alpha-synuclein (SNCA) protein - Optimal protein bioactivity, stability and reproducibility
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:<20 EU/mL,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:EM, FuncS, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Endogenous alpha-synuclein phosphorylation.100 μM alpha synuclein protein monomer (ab218818) seeded with 10 μM alpha synuclein protein aggregate (ab218819) in 25 μM Thioflavin T (ab120751) (PBS pH 7.4, 100 μl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 24 hours.Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a microplate reader.Endotoxin Level: 10-20 EU/mL,
Accession:P37840,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:Constituents: PBS

Product details:
Ensure the validity of your result using our Recombinant Alpha-synuclein (SNCA) protein
ab90285
as a control.
ab218819 can be used as a positive control in SDS-PAGE and western blot assays.
Check out our Thioflavin T assay protocol
For best results, sonicate ab218819 immediately prior to use. Sonication of PFFs is required in order to obtain fibrils with an average length of ~50nm, the optimal size for pathology.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.
Biological function summary
The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.
Pathways
Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.
Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.