Abcam, ab220044, Anti-ATP6J/ATP6V1G1 antibody

Size: 100µL
Rabbit Polyclonal ATP6J/ATP6V1G1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP6V1G1 aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ATP6V1G1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O75348

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ATP6J/ATP6V1G1 protein also known as V-type proton ATPase subunit G 1 is a component of the V1 domain of the vacuolar ATPase (V-ATPase) complex. The protein has a mass of roughly 13 kDa. It is expressed in various tissues with a higher abundance observed in the kidney and brain. The protein plays a role in the energy-dependent transport of protons across intracellular membranes an important component of cellular processes that require ATP hydrolysis.
Biological function summary
ATP6V1G1 is part of the multi-subunit V-ATPase complex which functions as a proton pump to acidify intracellular compartments like lysosomes and endosomes. The complex enables the maintenance of microenvironments necessary for processes such as protein sorting zymogen activation and receptor-mediated endocytosis. By facilitating pH regulation within cells ATP6V1G1 supports essential cellular activities and homeostasis.
Pathways
ATP6V1G1 integrates into the acidification pathway linked to intracellular organelles. It also interfaces with the mTOR signaling pathway which depends on lysosomal acidification for its activation. The cooperation between V-ATPase and mTOR relates to other binding proteins and complexes involved in cellular growth and autophagy such as LAMP2 and LC3.
Dysfunctions in ATP6V1G1-associated pathways contribute to lysosomal storage disorders and renal tubular acidosis. Impaired V-ATPase function affects lysosomal enzyme activation and cellular trafficking. Additionally altered interactions between ATP6V1G1 and cystinosin a protein involved in cystinosis link the V-ATPase to pathological states within the kidneys impacting cellular waste processing.