Abcam, ab220074, Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)]

Size: 100µL
Anti-BrdU antibody [BU1/75 (ICR1)] - Alexa Fluor® 488 conjugated (ab220074) is a rat monoclonal antibody detecting BrdU in ICC/IF .
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:BU1/75 (ICR1),
Isotype:IgG2a,
Conjugation:Alexa Fluor® 488,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdU incorporated into their DNA. Also reacts with chlorodeoxyuridine but with reduced staining. The antibody does not react with thymidine. It has been reported in the literature that this antibody clone cross-reacts with Edu (PMID: 23272138) and some customers reported that it cross reacts with IdU.

Product details:
What is this antibody validated in?
Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)] (ab220074) is a rat monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF).
Related products
Antibody clone BU1/75 (ICR1) is also available pre-conjugated to a variety of labels for your convenience – Anti-BrdU Alexa Fluor® 488 [BU1/75 (ICR1)] (ab220074).
Other related products
We have a range of other formats of antibody clone [BU1/75 (ICR1)] also available for your convenience:
ab6326
, Alexa Fluor® 488 - ab220074, Alexa Fluor® 647 -
ab220075
, Alexa Fluor® 594 -
ab220076
, HRP -
ab220507
, Alexa Fluor® 555 -
ab221240
, Carrier free -
ab264079
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
BrdU short for Bromodeoxyuridine is a synthetic analog of the nucleoside thymidine. It substitutes thymidine in newly synthesized DNA allowing for the tracking of cellular proliferation. Bromodeoxyuridine integrates into the DNA of proliferating cells which can then be detected using specific anti-BrdU antibodies. These antibodies are valuable tools in various assays such as BrdU staining and BrdU proliferation assay. BrdU has a molecular weight of approximately 315.13 g/mol. It is typically utilized in tissues or cell lines where active DNA synthesis and cellular proliferation are occurring.
Biological function summary
Bromodeoxyuridine acts as a marker for identifying cells in the S-phase of the cell cycle. During this phase DNA synthesis occurs and BrdU incorporates into the replicating DNA strand. When used in combination with anti-BrdU antibodies it enables researchers to identify and quantify dividing cells. This capability makes BrdU incorporation a widely accepted method to study cell proliferation in various biological samples. It functions independently without forming larger complexes.
Pathways
Bromodeoxyuridine integration highlights its role in DNA replication and repair pathways. It helps in the study of cell growth regulation pathways where proteins like PCNA (Proliferating Cell Nuclear Antigen) are involved. BrdU pairing with anti-BrdU antibodies allows assessment of DNA synthesis therefore aiding in the investigation of critical pathways like the cell cycle regulation pathway. In this context BrdU kits provide a consistent method for assessing dynamic changes in proliferative states.
Bromodeoxyuridine is useful in cancer research and neurological disorders like Alzheimer's disease. Through its ability to track cell proliferation researchers can explore cancerous tissue's growth patterns elucidating pathways and factors driving tumor expansion. BrdU assays contribute to understanding the aberrant cellular proliferation seen in tumors. For Alzheimer's disease BrdU aid in investigating neurogenesis rates particularly in regions affected by the disorder. Researchers often connect BrdU results with proteins like tau and amyloid-beta which play roles in these conditions.