Abcam, ab220117, Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3099] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RUNX1 / AML1 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, IHC-P, IP, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 11 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3099,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IP, IHC-P, WB, ChIC/CUT&RUN-seq, Flow Cyt (Intra), IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab220117 is the carrier-free version of
ab92336
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RUNX1 also known as AML1 along with RUNX2 and RUNX3 form a family of transcription factors that play a critical role in gene regulation. These proteins recognize specific DNA sequences through their Runt domain facilitating DNA binding and transcriptional activation or repression. RUNX1 is extensively studied having a mass of approximately 48 kDa. They are expressed in various tissues including hematopoietic cells osteoblasts and the central nervous system. RUNX proteins regulate genes involved in cell differentiation and proliferation.
Biological function summary
RUNX1 RUNX2 and RUNX3 control processes in development and differentiation. They often function as part of a transcriptional complex with CBFβ enhancing DNA binding and transcriptional activity. RUNX1 is vital in hematopoiesis guiding the formation of blood cells. RUNX2 predominantly influences bone development by regulating osteoblast differentiation while RUNX3 impacts neural development and T-cell maturation. These proteins collectively integrate signals to ensure proper cell lineage decisions.
Pathways
RUNX proteins participate in essential cellular pathways like the Wnt and TGF-β signaling pathways. The Wnt pathway involves interaction with β-catenin affecting cell fate and development processes. RUNX2 plays a significant role here by contributing to bone formation. The TGF-β pathway influences cell growth and differentiation by interacting with SMAD proteins primarily through RUNX3 which modulates immune responses and tumorigenesis. These pathways highlight RUNX proteins' critical integration in signaling networks.
RUNX1 mutations associate with various hematological malignancies notably acute myeloid leukemia (AML). RUNX1 mutations disrupt normal hematopoiesis leading to uncontrolled cell proliferation. RUNX2's dysregulation relates to cleidocranial dysplasia a skeletal disorder characterized by delayed bone development. Both RUNX1 and RUNX2 link to similar signaling pathways such as the Wnt pathway implicating them in these diseases' pathogenesis. Understanding RUNX proteins' role can lead to better diagnostic and therapeutic approaches for these conditions.