Abcam, ab22048, Anti-TLR4 antibody [76B357.1]

Size: 100µg
Anti-TLR4 antibody [76B357.1] (ab22048) is a mouse monoclonal antibody detecting TLR4 in Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF, ELISA . Suitable for Human, Mouse, Pig, Rat . - Over 290 publications - Trusted since 2006
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:76B357.1,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Rat, Human, Mouse, Pig,
Applications:ELISA, IHC-Fr, Flow Cyt (Intra), ICC, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human TLR4 aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.O00206,
Specificity:TLR4 expression levels and cleavage or degradation products can vary between different cell and tissue samples. Customers have observed this variability in WB band size and our laboratory has confirmed this variability as well observing lower molecular weight cleavage and degradation products and in some samples a lack of the full length TLR4 band. The TLR4 cleavage and degradation products and potential lack of full length TLR4 are well documented in the literature, including PMID 16885150 and 22927440. We recommend running a positive control human intestine tissue lysate.

Product details:
Product Specifications
Anti-TLR4 antibody [76B357.1] (ab22048) is a mouse monoclonal antibody and is validated for use in ELISA, Flow Cyt (Intra), ICC, ICC/IF, IHC-Fr, IHC-P in human, mouse, pig, rat samples.
Anti-TLR4 antibody [76B357.1] (ab22048) specifically detects TLR4 (UniProt ID: O00206; Molecular weight: 93kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-TLR4 antibody [76B357.1] (ab22048) has high sensitivity and specificity.
Anti-TLR4 antibody [76B357.1] (ab22048) has been cited over 297 times in peer reviewed journals and is trusted by the scientific community.
Anti-TLR4 antibody [76B357.1] (ab22048) has 26 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Toll-like receptor 4 (TLR4) is a membrane protein involved in the innate immune response. It serves as a receptor that recognizes pathogen-associated molecular patterns such as lipopolysaccharides from bacterial cell walls. Often referred to by its alternate names TLR-2 and CD284 this protein has a molecular weight of approximately 95 kDa. TLR4 is mainly expressed on the surface of immune cells like macrophages and dendritic cells enabling these cells to detect and respond to microbial invaders effectively.
Biological function summary
TLR4 plays an important role in activating the immune system. It forms a complex with myeloid differentiation factor 2 (MD-2) to facilitate binding to its ligands. Upon activation TLR4 triggers a signaling cascade that leads to the release of pro-inflammatory cytokines and interferons which are important for initiating the immune response. This early detection mechanism alerts the immune system to the presence of pathogens allowing the body to mount a defense.
Pathways
TLR4 participates in the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. These pathways are central to the inflammatory response with TLR4 acting as a sensor that activates downstream components like the MyD88 and TRIF proteins. These interactions result in the expression of genes that produce cytokines and enzymes involved in inflammation and immunity.
TLR4 has been implicated in the development of sepsis and atherosclerosis. In sepsis TLR4 recognizes lipopolysaccharides from bacterial infections which can cause excessive inflammation if uncontrolled. In atherosclerosis TLR4 receptors on vascular cells respond to endogenous ligands such as oxidized low-density lipoproteins contributing to plaque formation. The TLR4 interaction with proteins like CD14 and MD-2 is significant as it influences the inflammatory pathways that exacerbate these conditions.