Abcam, ab221545, Anti-DEK antibody [EPR20401]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal DEK antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20401,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra), ICC/IF, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DEK is a nuclear phosphoprotein with an approximate mass of 45 kDa. Researchers often study DEK in the context of its function in chromatin organization and transcription regulation as it serves as an architectural chromatin protein. DEK expression is ubiquitous found in various tissues with high levels in actively dividing cells. It is essential in processes that require dynamic chromatin remodeling.
Biological function summary
DEK plays a role in maintaining genomic integrity and regulating transcription. It operates within a complex that interacts with histone proteins to modulate chromatin structure. DEK also affects mRNA splicing by interacting with the spliceosomal complex further underlining its role in transcription control. As a multifunctional protein DEK helps coordinate several essential cellular activities.
Pathways
DEK integrates into the p53 and Wnt signaling pathways. It influences cell cycle control and apoptosis within the p53 pathway interacting with proteins like MDM2 to affect p53 stability. Within the Wnt pathway DEK contributes to cell proliferation and differentiation by affecting beta-catenin levels and interacting with transcription factors involved in Wnt target gene expression.
DEK has strong connections to various types of cancers and autoimmune diseases. DEK overexpression is often associated with tumorigenesis particularly in acute myeloid leukemia where it collaborates with NUP214 fusion partners. In autoimmune disorders like systemic lupus erythematosus DEK may become an autoantigen and its interaction with proteins such as nucleolin could contribute to disease pathogenesis.