Abcam, ab222872, Human Dipeptidyl Peptidase IV ELISA Kit (CD26)

Size: 1 x 96Tests
Human Dipeptidyl Peptidase IV ELISA Kit (CD26) is a Sandwich (quantitative) ELISA for the measurement of Human Dipeptidyl Peptidase IV (CD26) in Human in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.2 ng/mL,
Range:3.125 - 200 ng/mL,
Assay time:4h,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Dipeptidyl Peptidase IV ELISA Kit (CD26) (ab222872) is designed for the quantitative measurement of Dipeptidyl Peptidase IV (DPP4) in human plasma, serum cell lysate, tissue and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human DPP4 in less than 4 hours. A polyclonal antibody specific for human DPP4 has been pre-coated onto a 96-well microplate with removable strips. DPP4 in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for DPP4, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Dipeptidyl peptidase 4 (DPP4), also known as adenosine deaminase complexing protein 2 (ADCP-2), T-cell activation antigen CD26 or CD26, belongs to the peptidase S9B family and DPPIV subfamily. It is an intrinsic membrane glycoprotein that comprises 766 amino acids and weighs 110 kDa. Its extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity. Under certain stimuli and chronic inflammation, DPP4 can be released from the membrane, constituting a soluble form by matrix metalloproteases (MMPs). As a serine exopeptidase, DPP4 cleaves and inactivates N-terminal X-proline dipeptides of cytokines, chemokines, and neuropeptides involved in inflammation, immunity, and vascular function. Circulating soluble DPP4 is a novel adipokine that may impair insulin sensitivity in an autocrine and paracrine fashion. DPP4 plays a role in glucose homeostasis through proteolytic inactivation of the intestinal peptide incretins, such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide 1 (GLP1), which are major regulators of post-prandial insulin secretion.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dipeptidyl peptidase 4 also known as DPP4 or CD26 functions as a serine exopeptidase. This enzyme is involved in cleaving dipeptides from the N-terminus of polypeptides. DPP4 with a mass of approximately 110 kDa is expressed in various tissues such as the liver intestines kidney and lymphocytes. It exists in both a membrane-bound form and a soluble form in blood plasma.
Biological function summary
DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.
Pathways
DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.
DPP4 involvement is relevant in type 2 diabetes and immunological disorders. In type 2 diabetes DPP4 inhibitors modulate the incretin hormones improving glycemic control. Proteins like GLP-1 are affected by DPP4 activity connecting it to diabetes management. In autoimmune diseases altered DPP4 expression can impact immune cell functions influencing conditions such as rheumatoid arthritis. Through its effects on ADA and associated pathways DPP4 can influence both metabolic and immune-related disorders.