Abcam, ab223114, Anti-ADCK4 antibody

Size: 50µL
Rabbit Polyclonal ADCK4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COQ8B aa 1-150.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human COQ8B aa 1-150. The exact immunogen used to generate this antibody is proprietary information.Q96D53

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ADCK4 also known as aarF domain containing kinase 4 is a protein kinase with a molecular weight of approximately 49 kDa. It mainly exists in the mitochondria where it plays a significant role in cellular energy production. Expression of ADCK4 appears highest in the kidney and to a lesser extent in other tissues. As a kinase its mechanical function depends on phosphorylating various substrates which is necessary for the maintenance of mitochondrial function.
Biological function summary
ADCK4 participates in the biosynthesis of coenzyme Q10 a vital component of the electron transport chain. This protein forms part of a larger complex known as the coenzyme Q biosynthesis complex where it helps maintain cellular energy and metabolic balance. It interacts closely with other proteins involved in this pathway to regulate coenzyme Q10 levels which affects multiple cellular processes including ATP production.
Pathways
ADCK4 significantly integrates into the mitochondrial respiratory chain pathway and the broader metabolic energy pathway. It aids in the synthesis of coenzyme Q10 alongside proteins like COQ6 and COQ7 directly impacting the efficiency of energy production in mitochondria. This involvement supports metabolic activities that are critical for cellular homeostasis and energy regulation.
Mutations or dysfunctions in ADCK4 correlate with nephrotic syndrome and coenzyme Q10 deficiency. Individuals with nephrotic syndrome often show altered ADCK4 activity which affects coenzyme Q10 synthesis and leads to kidney-related complications. Additionally connections between ADCK4 and the action of COQ2 indicate potential pathways through which mitochondrial and renal disorders might develop from inadequate coenzyme Q10 levels.