Abcam, ab223594, Human TSC-1 ELISA Kit

Size: 1 x 96Tests
Human TSC-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TSC-1 with a sensitivity of 1.54 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.54 pg/mL,
Range:4.69 - 300 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human TSC-1 ELISA Kit (ab223594) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TSC-1 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TSC-1 with 1.54 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
TSC-1 (Eotaxin 3 / CCL26) is a small cytokine within the CC chemokine family coded by a gene located on human chromosome 7. TSC-1 is also know by Macrophage inflammatory protein 4-alpha, Thymic stroma chemokine-1 and IMAC. It has known expression in heart, lung and ovarian tissue. TSC-1 expression can be induced by IL-4 stimulation which then can activate chemotaxic activity of eosinophils and basophiles by binding to the cell surface chemokine receptor CCR3.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tuberous Sclerosis Complex 1 (TSC-1) also known as hamartin is a protein with a molecular mass of approximately 130 kDa. It plays a direct role in cellular signaling and regulatory functions. TSC-1 is expressed broadly across tissues which suggests its importance in maintaining normal cellular physiology. It interfaces with various signaling molecules and directly interacts with TSC-2 to form a protein complex.
Biological function summary
TSC-1 plays a regulatory role in cell growth and proliferation. It forms a complex with TSC-2 also known as tuberin which is important for its function. This complex acts as a GAP (GTPase-activating protein) toward Rheb a small GTPase inhibiting the mTOR signaling pathway. The proper regulation of this pathway ensures controlled cell size and growth emphasizing the importance of the TSC-1/TSC-2 complex in cellular regulation.
Pathways
TSC-1 operates within the mTOR signaling pathway to influence cellular processes like autophagy and protein synthesis. It also relates to the PI3K/AKT pathway one that is involved in the regulation of cell survival and metabolism. By interacting with TSC-2 in these pathways TSC-1 modulates the inhibition of mTORC1 thereby impacting cellular homeostasis and growth.
Defects in TSC-1 can lead to tuberous sclerosis complex a condition characterized by benign tumors in multiple organs. Mutations in TSC-1 and its partner TSC-2 disrupt normal cellular functions leading to this disease. Additionally aberrations in the mTOR pathway influenced by TSC-1 malfunction have associations with certain cancers highlighting the importance of TSC-1 in maintaining cell proliferation and signaling balance.