Abcam, ab223779, Anti-ATPase Inhibitory Factor 1/IF1 antibody

Size: 100µL
Rabbit Polyclonal ATPase Inhibitory Factor 1/IF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATP5IF1 aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ATP5IF1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9UII2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATPase Inhibitory Factor 1 (IF1) also referred to as IF1 protein ATPIF1 or ATPase inhibitory factor 1 is a small protein with a mass of approximately 12 kDa. It is expressed primarily in mitochondria-rich tissues such as the heart liver and brain where it plays an important role in energy metabolism. IF1 is notable for its ability to bind to the F1 subunit of the mitochondrial ATP synthase inhibiting its ATPase activity under specific conditions.
Biological function summary
IF1 influences mitochondrial function by regulating ATP synthase in response to changes in the cellular environment. Under conditions of low cellular energy IF1 stabilizes and prevents hydrolysis of ATP by binding to the F1 catalytic domain of the enzyme. This regulation helps to conserve ATP critical for maintaining cellular viability during stress. IF1 can also interact with other mitochondrial proteins to form complexes that influence energy homeostasis in the cell.
Pathways
IF1 integrates into pathways involved in cellular energy management including oxidative phosphorylation and ATP synthesis pathways. It interacts significantly with proteins such as the mitochondrial F1Fo-ATP synthase complex. By modulating ATP synthase activity IF1 ensures efficient ATP production and maintains mitochondrial membrane potential important for proper cell function.
IF1 has associations with metabolic conditions and cancer. Abnormal expression or activity of IF1 correlates with mitochondrial dysfunction often observed in cancer cells where it affects cell proliferation. Additionally IF1 has links to cardiac conditions given its role in regulating heart metabolism. It relates to proteins like the cytochrome c oxidase influencing cellular respiration and affecting disease progression.