Abcam, ab223877, Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal DFNA5/GSDME antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19859,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Our previous testing data indicates that this antibody detects no signal or very weak signal in mouse heart, spleen, lung, stomach, testicle, skin, pancreas, muscle and lymph node tissues. Final results may be affected by the expression level of the tested samples.

Product details:
ab223877 is the carrier-free version of
ab215191
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DFNA5 also known as GSDME (Gasdermin E) is a protein encoded by the GSDME gene. It has a molecular mass of approximately 59 kDa. The protein is expressed in various tissues including the cochlea in the inner ear and some epithelial tissues. Mechanically DFNA5/GSDME plays a role in inducing cell membrane pore formation leading to cell lysis. This activity relates to its involvement in processes like cell death specifically pyroptosis where the cell undergoes a form of programmed necrosis.
Biological function summary
DFNA5/GSDME serves as a pore-forming protein that facilitates pyroptotic cell death usually upon cleavage by caspase-3. This activity is important for innate immune response and the maintenance of cellular homeostasis. DFNA5/GSDME operates independently and does not form part of large protein complexes. The cleaved form inserts into the lipid bilayer of cell membranes contributing to the execution of cell death particularly under stress conditions or cellular insult.
Pathways
DFNA5/GSDME is involved in the pyroptosis and apoptosis pathways. It plays an essential role in the cellular response to inflammation and stress signals by interacting with caspase-3 a critical protease in the apoptosis pathway. The balance between apoptosis and pyroptosis decides cell fate with DFNA5/GSDME activation tipping towards pyroptotic cell death. The interconnection with other gasdermin family proteins such as GSDMD is notable as they share functional and structural similarities in promoting necrosis-like cell death processes.
Mutations in the DFNA5 gene relate to progressive hearing loss (nonsyndromic sensorineural deafness). Dysregulation of GSDME has also been implicated in cancer where altered expression promotes tumorigenesis or tumor suppression depending on the context. In hearing loss GSDME's interaction with pathways influencing apoptosis highlights its pathogenic role while in cancer connections to caspase-3 show a potential dichotomy in cell survival and cell death regulation.