Abcam, ab224539, Anti-FIP1L1 antibody

Size: 100µL
Rabbit Polyclonal FIP1L1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FIP1L1 aa 250-400.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human FIP1L1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.Q6UN15

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein FIP1L1 also known as Factor Interacting with PAPOLA and CPSF1 is a component of the mRNA processing machinery. It plays a vital role in polyadenylation an important step in mRNA processing. The protein is approximately 68 kDa in mass and is expressed in various tissues with significant levels in hematopoietic cells. This expression pattern suggests that FIP1L1 participates in processes requiring active mRNA synthesis and processing.
Biological function summary
The actions of FIP1L1 modify the mRNA polyadenylation process essential for mRNA stability and export from the nucleus. FIP1L1 acts as part of the Cleavage and Polyadenylation Specificity Factor (CPSF) complex. This complex determines the site of polyadenylation in pre-mRNA integrating signals essential for proper mRNA maturation. FIP1L1's function in this complex showcases its role in gene expression regulation and cellular messaging.
Pathways
FIP1L1 integrates into transcriptional and post-transcriptional pathways affecting mRNA processing. It interacts notably with the polyadenylation signal recognition pathway contributing to the control of RNA processing and the stability of mRNA transcripts. FIP1L1's interaction with proteins such as CPSF complex components ensures effective communications and transitions between transcriptional machinery and mRNA processing mechanisms.
FIP1L1 links especially to eosinophilic disorders including chronic eosinophilic leukemia. A small deletion in chromosome 4q12 can produce a fusion gene between FIP1L1 and PDGFRA leading to the constitutive activation of this receptor tyrosine kinase; this fusion is significant in the pathogenesis of the disease. Understanding FIP1L1’s role in this fusion helps in delineating therapeutic targets for related hematological diseases.