Abcam, ab225878, Anti-CCT3 antibody

Size: 100µL
Rabbit Polyclonal CCT3 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CCT3 aa 300-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CCT3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.P49368

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab225878 was affinity purified using an epitope specific to CCT3 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CCT3 also known as TCP-1-γ is a subunit of the chaperonin-containing TCP1 (CCT) complex also called the TRiC complex. This protein has a molecular mass of approximately 60 kDa and is expressed in many tissues but is especially abundant in the cytoplasm of eukaryotic cells. CCT3 plays an essential role in the folding of actin and tubulin which are critical for maintaining the cell's cytoskeleton structure. The mechanical function of CCT3 includes providing a folding chamber where newly synthesized polypeptides achieve their correct conformation preventing aggregation and ensuring functional protein production.
Biological function summary
CCT3 is a component of the TRiC/CCT complex which is important for protein quality control within the cell. This complex assists in folding a subset of proteins that are typically difficult to fold without help. CCT3 collaborates with other subunits in the complex to ensure proper assembly and function of structural proteins. Its role is particularly important in high turnover tissues or rapidly proliferating cells where efficient protein folding is essential to maintain cellular function and growth.
Pathways
CCT3 is involved in essential signaling cascades like the cytoskeleton regulation and protein homeostasis pathways. It has relationships with proteins such as actin and beta-tubulin which are critical parts of these pathways. The accurate folding facilitated by CCT3 influences the stability and function of these proteins impacting cell division motility and organization. The involvement in these pathways highlights CCT3’s contribution to maintaining cellular integrity and function.
Mutations or abnormal expression of CCT3 have been linked to neurodegenerative disorders and certain cancers. Dysregulation of this protein may contribute to amyotrophic lateral sclerosis (ALS) due to its role in protein homeostasis and cytoskeleton maintenance. In cancer overexpression of CCT3 has been observed in conditions such as hepatocellular carcinoma potentially promoting tumor growth through altered protein folding. The interaction of CCT3 with proteins like HSP70 during stress responses also connects it to processes that might protect against disease-related protein aggregation.