Abcam, ab226049, Anti-HP1 alpha antibody [EPR5777] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HP1 alpha antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Rat, Human, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5777,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, Flow Cyt (Intra), IHC-P, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab226049 is the carrier-free version of
ab109028
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HP1 alpha also known as HP1a or HP1 protein is a well-studied member of the heterochromatin protein 1 family. It plays a mechanical role in chromatin organization and gene regulation by binding to histone H3 when it's methylated at lysine 9 (H3K9me). This protein has a molecular mass of approximately 22-25 kDa. HP1 alpha is commonly expressed in the nuclei of eukaryotic cells where it localizes to heterochromatin regions and influences chromatin compaction.
Biological function summary
Heterochromatin protein 1 alpha acts as a modulator of gene silencing. It is part of a larger protein complex that contains various chromatin modifiers responsible for maintaining the silent state of certain genes. By mediating these interactions HP1 alpha helps regulate transcription replication timing and DNA repair processes. This regulation is essential for the structural maintenance of heterochromatin and plays a part in chromosomal stability.
Pathways
HP1 alpha is integral in the epigenetic regulation pathway along with other well-known proteins including SUV39H1 and SUV39H2 which are histone methyltransferases. HP1 alpha also links to the DNA damage response pathway where it recruits repair factors to maintain genomic integrity. Through these pathways HP1 alpha interacts closely with proteins like BRCA1 emphasizing its role in cellular homeostasis and response to DNA damage.
Researchers associate HP1 alpha with cancer and developmental disorders. Alterations in HP1 alpha expression or function can lead to disruptions in chromatin organization contributing to oncogenesis by affecting tumor suppressor genes. Furthermore HP1 alpha links to disorders like ICF syndrome through its relationship with the DNA methylation process where it interacts with proteins such as DNMT3B. Understanding these connections provides insights into therapeutic approaches targeting epigenetic modifications in disease contexts.