Abcam, ab226187, Anti-ADK antibody - C-terminal

Size: 100µL
Rabbit Polyclonal ADK antibody. C-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ADK aa 300 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ADK aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P55263

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab226187 was affinity purified using an epitope specific to ADK immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Adenosine kinase (ADK) also known by its alternative name ATP:adenosine 5'-phosphotransferase is an enzyme with a molecular mass of approximately 40 kDa. This enzyme catalyzes the transfer of a phosphate group from ATP to adenosine forming AMP. ADK is expressed in several tissues with significant presence in the liver brain and lungs. This distribution suggests its versatile role in organ-specific metabolism and regulatory processes.
Biological function summary
The enzyme modulates cellular adenosine levels which influences various physiological functions such as sleep regulation and immune responses. ADK does not operate as part of a larger protein complex but acts independently to maintain intracellular energy balance by regulating the pools of adenosine and AMP. The enzyme activity affects purine metabolism important in diverse cellular activities.
Pathways
The activity of adenosine kinase fits prominently into the purine metabolism and energy homeostasis pathways. Its function maintains the cellular concentrations of adenosine a nucleotide affecting cellular signaling pathways including the AMP-activated protein kinase (AMPK) pathway. ADK interacts with enzymes like adenosine deaminase which collectively orchestrate the purine salvage pathway—pivotal in nucleotide recycling.
Irregularities in ADK function have implications in neurological disorders and liver diseases. Abnormal ADK activity links to epilepsy due to its role in controlling adenosine concentrations affecting neurotransmitter release. Liver diseases such as hepatic steatosis have shown associations with altered ADK function impacting energy balance. The interplay with proteins such as AMP-activated protein kinase indicates potential therapeutic targets in disorders where metabolic regulation is compromised.