Abcam, ab226899, Human Alpha-1-Microglobulin ELISA Kit

Size: 1 x 96Tests
Human Alpha-1-Microglobulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Alpha-1-Microglobulin with a sensitivity of 725 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Urine, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 725 pg/mL,
Range:1.56 - 100 ng/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Alpha-1-Microglobulin ELISA Kit (ab226899) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Alpha-1-Microglobulin protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Alpha-1-Microglobulin with 725 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Alpha 1-microglobulin is expressed in the liver and secreted in the plasma acting as a radical scavenger and degrades heme. Alpha 1-Microglobulin can exist as either a monomer or in complexes with IgA or Albumin. The precursor to Alpha 1-microglobulin (Protein AMBP) is cleaved into three separate functioning proteins (Alpha 1-microglobulin, Bikunin, and Trypstatin). ab226899 is designed to recognize Alpha-1-microglobin, specificity over the pre-cursor has not been tested.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha-1-microglobulin also known as alpha-1-microglycoprotein or A1M is a protein with a mass of approximately 26 kDa. It is mainly expressed in the liver and secreted into the blood plasma. Alpha-1-microglobulin can bind to small peptides and free radicals acting as a scavenger. It exists in a monomeric form and is present in various bodily fluids including urine and amniotic fluid.
Biological function summary
This protein serves as a protective factor against oxidative stress. Alpha-1-microglobulin is part of the lipocalin protein family which has roles in transport of small hydrophobic molecules in extracellular spaces. Its antioxidant properties help protect biological macromolecules from damage induced by oxidative agents. Alpha-1-microglobulin contains heme-binding sites which contribute to its protective biochemical actions.
Pathways
Alpha-1-microglobulin participates in oxidative stress-related and inflammatory pathways. Within the oxidative stress pathway it interacts with heme oxygenase and other heme-binding proteins to mitigate toxic effects of excess free heme. In inflammatory pathways it links with proteins such as albumin and immunoglobulin components which further supports immune response and cellular protection mechanisms.
Alpha-1-microglobulin has associations with conditions like preeclampsia and kidney disease. In preeclampsia its levels can indicate oxidative stress and placental dysfunction involving interactions with other proteins such as haptoglobin. In kidney disease altered levels of alpha-1-microglobulin in urine serve as a biomarker for tubular dysfunction connecting it to conditions affecting renal function and health.