Abcam, ab227685, Anti-SATB1 antibody [SP280] - C-terminal

Size: 100µL / 500µL / 1mL
Rabbit Recombinant Monoclonal SATB1 antibody. C-terminal. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP280,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Purification notes-Purified from TCS by protein A/G., Storage buffer-pH: 7.6Preservative: 0.099% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SATB1 or Special AT-rich Sequence Binding Protein 1 is a nuclear matrix-associated protein with a mass of approximately 85 kDa. It modifies chromatin structure by organizing the density of DNA through loop formation which regulates gene expression. SATB1 is expressed in thymocytes breast tissue and various other cell types and plays a significant role in cellular differentiation and development. It binds selectively to base-unpairing regions allowing it to influence expression patterns of multiple genes.
Biological function summary
SATB1 acts as a genome organizer and transcription regulator by linking chromatin remodeling complexes to specific genomic loci. It participates in chromatin loop domain organization influencing higher-order chromatin structure and gene silencing or activation. SATB1 does not usually work alone but rather associates with corepressors such as HDAC1 and coactivators like PCAF in regulating transcription. Its functions have been noted as particularly important in T cell development where it assists in the regulation of T cell receptor genes.
Pathways
SATB1 integrates into the Wnt signaling pathway and influences the Notch signaling pathway. The protein interacts with β-catenin in the Wnt pathway aiding in sustaining key developmental processes. In the Notch signaling pathway SATB1 influences cell fate decisions impacting differentiation and proliferation through its interaction with RBP-Jκ and other transcription modulators. MUTYH and LRP6 are among the proteins within these pathways influenced by SATB1's regulatory capacity.
SATB1 links to various cancer types including breast cancer and colorectal cancer. Overexpression of SATB1 can drive tumor progression and metastasis by altering gene expression landscapes. It's known to affect pathways regulated by proteins like BRCA1 in breast cancer contributing to disease pathogenicity. In immune disorders such as autoimmune diseases SATB1 interacts with STAT5 impacting cytokine signaling pathways and thereby influencing immunological tolerance and efficacy.