Abcam, ab227707, Anti-PAX8 antibody [SP348] - N-terminal

Size: 20µL / 100µL / 500µL / 1mL
Anti-PAX8 antibody [SP348] - N-terminal (ab227707) is a rabbit monoclonal antibody detecting PAX8 in Western Blot, Flow Cytometry, IHC-P, ICC/IF, mIHC . Suitable for Human . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:SP348,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, mIHC, WB, ChIC/CUT&RUN-seq, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Anti-PAX8 antibody [SP348] - N-terminal (ab227707) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, Flow Cyt ICC/IF, IHC-P, WB and mIHC.
Abcam's high quality manufacturing and validation processes ensure Anti-PAX8 antibody [SP348] - N-terminal (ab227707) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-PAX8 antibody [SP348] - N-terminal (ab227707) specifically detects PAX8 (UniProt ID: Q06710; Molecular weight: 49kDa) and is sold in 100 µL, 500 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone SP348 -
ab242429
Antibody clone SP348 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
ab313345
Anti-PAX8 antibody [SP348] - N-terminal (ab227707) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).
Highly-specific to PAX8. Western blotting data to guarantee specificity to PAX8 with no cross-reactivity with other PAX family members. Validated for CUT&RUN-seq, a key application to map protein-DNA interactions on a genome-wide scale using NGS. A highly-validated antibody for studying PAX8 as a transcription factor, its role in thyroid and renal cancers and epigenetic regulation. This antibody is crucial in cancer research, particularly in understanding PAX8's involvement in tumorigenesis and gene expression and is widely used in studies of embryogenesis and cancers of the Mьllerian duct.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Müllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.
Biological function summary
The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.
Pathways
PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.
PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.