Abcam, ab227908, Anti-Necdin antibody

Size: 100µg
Rabbit Polyclonal Necdin antibody. Suitable for WB, IHC-Fr and reacts with Mouse samples. Cited in 21 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ndn aa 50 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WB, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Mouse Ndn aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P25233

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 6.5 - 8.5Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Necdin also known simply as NDN is a protein playing an important mechanical role in cellular functioning. It has a molecular mass of approximately 33 kDa. Necdin is expressed widely in postmitotic neurons in the brain and spinal cord where it contributes to the proper functioning of these cells. The protein also appears in other tissues but at notably lower levels. Necdin primarily functions as a transcriptional repressor that interacts with other molecules to regulate gene expression.
Biological function summary
Necdin carries out its function by forming complexes with other proteins such as p53 and E2F1 which regulate the cell cycle and apoptosis. Through interacting with these complexes Necdin modulates transcriptional activities that affect neuronal development and differentiation. Besides its role in neurons Necdin’s involvement in other cell types suggests it has a broader impact on cell proliferation and survival.
Pathways
Necdin fits significantly into the cell survival and apoptosis pathways. It interacts with p53 an important protein in the apoptosis pathway regulating cell death and preventing uncontrolled cell proliferation. Necdin also associates with the Retinoblastoma protein (Rb) pathway through E2F1 where it influences cell cycle progression. These interactions highlight its contribution to maintaining physiological balance and preventing aberrant cellular processes.
Necdin shows a connection to conditions like Prader-Willi Syndrome (PWS) and certain cancers. PWS caused by the absence of paternal Necdin expression results from deletions or disruptions in chromosome 15q11-q13 which impacts the development of the nervous and endocrine systems. Necdin's interaction with proteins like E2F1 highlights its involvement in cancer progression where dysregulation affects cell cycle control. Therefore Necdin's function and interaction with other proteins have significant implications for both developmental and pathological conditions.