Abcam, ab227995, Anti-p38 beta/MAPK11 + p38 alpha/MAPK14 antibody [Y122] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal p38 beta/MAPK11 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:Y122,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody recognises p38. It is predicted to react with splice isoform CSBP1 according to sequence homology. Mouse cross reactivity has been tested by WB and IHC, Rat cross reactivity by WB only.

Product details:
ab227995 is the carrier-free version of
ab32142
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The proteins p38 beta (MAPK11) and p38 alpha (MAPK14) are important components in the mitogen-activated protein kinase (MAPK) family. They are involved in cellular responses to stress signals and are key players in signal transduction. p38 alpha also known as SAPK2a has a molecular weight of approximately 38 kDa. These proteins are ubiquitously expressed but show higher activity in tissues like the heart brain and skeletal muscles. They regulate gene expression by phosphorylating various transcription factors and substrates.
Biological function summary
P38 beta and p38 alpha functions include regulation of inflammation cell differentiation and apoptosis. They operate as part of a larger protein kinase complex and are activated by environmental stressors such as cytokines and UV radiation. This activation leads to a cascade of downstream signaling events modulating cellular processes essential for maintaining homeostasis. They have significant roles in immune response and stress-induced transcriptional regulation.
Pathways
These proteins primarily contribute to the MAPK signaling pathway and the stress-activated protein kinase (SAPK) pathway. The MAPK pathway also includes other kinases like JNK and ERK which work together to regulate cellular responses to a diverse array of stimuli. In the context of immune response these proteins play a critical role working in tandem with other kinases to facilitate cellular adaptation to stress and inflammation.
P38 beta and p38 alpha show significant involvement in inflammatory diseases and cancer. Their role in modulating inflammation links them to conditions such as rheumatoid arthritis where they regulate cytokine production and inflammatory response. Additionally dysregulation in the p38 signaling pathway has associations with cancer progression often interacting with proteins like NF-kB to influence tumorigenesis and cancer cell proliferation.