Abcam, ab228461, Anti-BRAF (mutated V600E) antibody [VE1]

Size: 100µL / 500µL / 1mL
Anti-BRAF (mutated V600E) antibody [VE1] (ab228461) is a mouse monoclonal antibody detecting BRAF in Western Blot, IHC-P . Suitable for Human . - Biophysical QC for unrivalled batch-batch consistency - Over 110 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:VE1,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The VE1 monoclonal is a sensitive antibody that detects mutated, constitutively active BRAF protein where glutamic acid is present at codon 600 instead of valine (V600E) (PubMed IDs: 21638088, 23657789).Please be aware that non-specific nuclear staining has been reported with this antibody (PubMed IDs: 23763264, 23589031, 24838325).

Product details:
Anti-BRAF (mutated V600E) antibody [VE1] (ab228461) is a mouse recombinant monoclonal antibody and is validated for use in IHC-P and WB.
Anti-BRAF (mutated V600E) antibody [VE1] (ab228461) was first used in a scientific publication in 2011 and has been cited over 116 times in peer reviewed journals. It's performance in IHC in human and mouse samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-BRAF (mutated V600E) antibody [VE1] (ab228461) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-BRAF (mutated V600E) antibody [VE1] (ab228461) has 6 independent reviews from customers.
Anti-BRAF (mutated V600E) antibody [VE1] (ab228461) specifically detects BRAF Mutated-V600E (UniProt ID: P15056; Molecular weight: 85kDa) and is sold in 100 µL, 500 µL and 1 mL selling sizes.
The BRAF gene helps control cell growth, mutations in BRAF can lead to uncontrolled cell division contributing to cancer. These mutations are common in cancers like melanoma, colon and thyroid, targeted therapies have been developed to treat cancers with BRAF mutations.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from TCS by protein A/G., Storage buffer-pH: 7.5Preservative: 0.1% Sodium azideConstituents: Tris, 0.3% Carrier protein, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
BRAF also known as v-Raf murine sarcoma viral oncogene homolog B1 is a protein kinase with a molecular weight of approximately 84 kDa. It is part of the RAF family of kinases which includes A-RAF and C-RAF. BRAF is expressed in various tissues with high expression levels in brain and testis. The protein functions as a serine/threonine kinase pivotal in transmitting signals from the extracellular space to the nucleus therefore influencing cell proliferation differentiation and survival.
Biological function summary
BRAF plays a central role in the MAPK/ERK signaling pathway which regulates cell cycle progression and apoptosis. It forms a part of a complex with other proteins such as RAS following activation. Mutations in BRAF including the common BRAF V600E result in constitutive activation of the pathway leading to uncontrolled cellular processes. This mutated form alters normal signaling enhancing its oncogenic potential.
Pathways
BRAF integrates into the MAPK/ERK and PI3K/AKT pathways which are essential for proper cellular signaling and function. The MAPK/ERK pathway in particular involves cooperation with proteins like MEK and ERK facilitating cellular responses to growth signals. Variants like BRAF V600K and BRAF V600E significantly impact these pathways by aberrantly activating downstream targets without external stimuli promoting oncogenesis.
BRAF mutations are critically implicated in melanoma and colorectal cancer. The BRAF V600E mutation for example occurs commonly in melanomas resulting in excessive signaling that drives tumorigenesis. BRAF also connects with proteins such as NRAS and KRAS through these cancers highlighting its role in the broader network of oncogenic signaling. Effective targeting of BRAF and its mutations represents an important strategy in cancer therapy.