Abcam, ab229246, Anti-KLHL25 antibody - C-terminal

Size: 100µL
Rabbit Polyclonal KLHL25 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KLHL25.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human KLHL25. The exact immunogen used to generate this antibody is proprietary information.Q9H0H3

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein KLHL25 also known as Kelch-like protein 25 is a member of the Kelch-like (KLHL) family. It has a mass of approximately 64 kDa. KLHL25 contains Kelch domains that participate in protein-protein interactions by forming beta-propeller structures. Expressed in various tissues including the brain liver and kidney KLHL25 functions as a substrate-specific adapter within E3 ubiquitin ligase complexes targeting specific proteins for ubiquitination and subsequent degradation by the proteasome.
Biological function summary
KLHL25 interacts with target proteins through its Kelch domains facilitating their ubiquitination as part of an E3 ligase complex. It participates in the fine-tuning of cellular processes by controlling protein turnover. Additionally KLHL25 plays a role in cytoskeletal organization by interacting with actin-binding proteins. These functions contribute to maintaining cellular homeostasis impacting cell signaling and structural integrity.
Pathways
KLHL25 integrates into cellular ubiquitination pathways that govern protein degradation. Its interaction affects signaling pathways such as the Wnt signaling pathway which is important for cell development and growth. KLHL25 likely works in concert with other E3 ligase components including CUL3 a scaffold protein that helps in forming multi-protein E3 ligase assemblies. The combined actions within these pathways help maintain balance in the presence of cellular stress or growth signals.
KLHL25 links to neurodegenerative diseases one being Parkinson’s disease through its role in protein degradation pathways. It potentially affects the stability of proteins like alpha-synuclein which aggregates in Parkinson's disease. Abnormal function or expression of KLHL25 may contribute to the accumulation of misfolded proteins disrupting normal cell function and leading to neurodegeneration. Understanding KLHL25’s role in these contexts could offer insights into therapeutic strategies for managing such conditions.