Abcam, ab229392, Human Lactoferrin ELISA Kit, Fluorescent

Size: 1 x 96Tests
Human Lactoferrin ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Lactoferrin with a sensitivity of 17.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
Detection method:Fluorescent,
Sample types:Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 17.7 pg/mL,
Range:19.5 - 80000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Lactoferrin
in vitro
CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Lactoferrin protein in human serum, plasma, milk, urine, saliva, and cell culture supernatants.
This CatchPoint SimpleStep ELISA kit has been
optimized for Molecular Devices Microplate Readers
. Click
for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at
www.softmaxpro.org
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Lactoferrin is a member of the transferrin family and is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. High levels of Lactoferrin are found in saliva and tears, intermediate levels in serum and plasma, and low levels in urine. The canonical sequence of Lactoferrin (known as isoform 1) is 710 amino acids in length and runs at 78.2 kDa. Isoform Delta-Lactoferrin (or DeltaLF) is missing amino acids 1 – 44 of the canonical sequence. Mouse and rat Lactoferrin are 71% and 62% identical to human Lactoferrin, respectively.
Lactoferrin possesses antibacterial, antiviral, anti-inflammatory, and immunoregulatory properties. The presence of Lactoferrin in neutrophils and the release of Lactoferrin during inflammation suggest that Lactoferrin is involved in phagocytic killing and immune responses. Additionally, Lactoferrin mediates bacteriostasis through its ability to sequester free iron and inhibit microbial growth. Lactoferrin also directs bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. Furthermore, Lactoferrin may play a role in the regulation of bone growth because it has anabolic, differentiating, and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis. Salivary Lactoferrin plays an important role in protecting the oral cavity against several oral pathogens including Streptococcus mutans. Additionally, increased levels of Lactoferrin in fecal samples is used as a marker of active inflammatory bowel disease (IBD).

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lactoferrin also known as lactotransferrin is an iron-binding glycoprotein with a molecular weight of about 80 kDa. It predominantly exists in mammalian milk particularly in high concentrations in colostrum and is also found in various epithelial secretions such as saliva tears and nasal fluids. The liver and neutrophils also produce lactoferrin and it plays a significant role in the body's primary defense system. As an antibacterial agent lactoferrin sequesters free iron to limit bacterial growth and disrupt biofilms assisting in the protection against microbial invasions.
Biological function summary
Lactoferrin exhibits multiple functions beyond its antimicrobial properties. It contributes to immunomodulation by binding to specific receptors on immune cells enhancing phagocytosis and influencing cytokine production. Also it possesses anti-inflammatory and antioxidant properties. Lactoferrin interacts with cellular components including glycosaminoglycans and lipoproteins impacting the activity and expression of proteins within signaling and metabolic pathways. This multifaceted role places it as a critical participant in both innate and adaptive immune responses although it does not form part of a stable protein complex.
Pathways
Lactoferrin plays a significant role in the iron metabolism and immune response pathways. It tightly interacts with the transferrin receptor modulating iron uptake and distribution across tissues which helps prevent iron overload and oxidative stress. Furthermore lactoferrin links with the toll-like receptor pathways particularly TLR4 which enables the modulation of innate immune responses against pathogens. By influencing nuclear factor kappa B (NF-kB) signaling lactoferrin impacts inflammation and immune responses working together with proteins such as ferritin and hepcidin.
Lactoferrin has relevancy to diseases like anemia of chronic disease and inflammatory bowel disease (IBD). Its role in regulating iron homeostasis makes it essential for preventing anemia associated with chronic inflammation. Lactoferrin mitigates dysregulated immune responses in IBD potentially reducing inflammation and promoting gut health. Disease-modifying properties of lactoferrin connect it to glycoproteins like transferrin and inflammatory mediators demonstrating its potential therapeutic importance.