Abcam, ab229474, Anti-LIM1/LHX1 antibody

Size: 100µL
Rabbit Polyclonal LIM1/LHX1 antibody. Suitable for ICC/IF and reacts with Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LHX1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human LHX1. The exact immunogen used to generate this antibody is proprietary information.P48742

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.025% Proclin 300Constituents: PBS, 20% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LIM1 also known as LHX1 is a member of the LIM homeodomain protein family. Its role involves transcriptional regulation of genes necessary for embryonic development. LIM1 contains a LIM domain—a double zinc finger structure—that allows for protein-protein interactions and a homeodomain necessary for DNA binding. With an approximate mass of 44 kilodaltons LIM1 is expressed in various tissues including the developing brain kidneys and reproductive organs. This multifaceted expression points to its involvement in multiple developmental processes.
Biological function summary
LIM1 serves as a regulator of gene expression critical for proper tissue and organ formation. During early embryogenesis it coordinates the development of structures in the central nervous system and urogenital systems. LIM1 often functions within a transcriptional complex involving other proteins facilitating the activation or repression of target gene transcription. This action is important for the precise spatial and temporal patterning required for organ and tissue differentiation.
Pathways
LIM1 integrates into several signaling pathways that coordinate developmental cues. Two significant pathways include the Wnt and BMP pathways. Within these LIM1 interacts with other key proteins like β-catenin and Smad proteins ensuring proper transmission of developmental signals. These pathways underpin the processes of pattern formation and axis specification positioning LIM1 as a central regulatory node within these systems.
LIM1 has been associated with congenital disorders such as renal malformations and various neurological deficits. Abnormal expression or function of LIM1 can disrupt the developmental processes controlled by its pathways. For instance its interaction with BMP signaling is related to certain nephrogenic disorders. Disorders of the central nervous system have also linked malfunctions with other LIM homeodomain proteins highlighting the broader impact of this protein family on development and disease.