Abcam, ab229701, Anti-SNAIL antibody [EPR21043] - BSA and Azide free

Size: 100µg / 1mg
Knockout Tested Rabbit Recombinant Monoclonal SNAIL antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 27 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21043,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab229701 is the carrier-free version of
ab216347
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SNAIL also known as SNAI1 is a zinc finger transcription factor involved in the regulation of cellular processes. The SNAIL protein has a mass of approximately 29 kDa and is robustly expressed in various tissues including embryonic tissues and cancerous cells. The protein functions as a repressor of transcription influencing the expression of genes associated with cellular adhesion and movement. Due to its integral role SNAIL is involved in complex regulatory networks that control cell fate and differentiation.
Biological function summary
SNAIL contributes to the epithelial-mesenchymal transition (EMT) a process critical for embryogenesis and tumor progression. It forms a part of a complex network of transcription factors that regulate cell-cell adhesion molecules like E-cadherin. SNAIL's ability to bind to specific DNA sequences allows it to suppress or activate the transcription of target genes promoting cellular metamorphosis and enabling cells to acquire motility and invade other tissues.
Pathways
SNAIL operates significantly within the TGF-beta and Wnt signaling pathways. The TGF-beta pathway enhances the expression of SNAIL which in turn represses genes that maintain the epithelial phenotype. The Wnt pathway also modulates SNAIL activity connecting it with proteins such as beta-catenin to drive EMT. These pathways highlight SNAIL's involvement in sophisticated signaling pathways that determine cell behavior adaptation and tissue remodeling.
Overexpression of SNAIL associates with cancer progression particularly in metastasis due to its role in EMT. It connects with proteins like Slug and ZEB1 in this context enhancing the invasive capabilities of cancer cells. Moreover SNAIL is implicated in fibrotic diseases where excessive tissue scarring occurs. In such conditions TGF-beta-mediated activation of SNAIL contributes to abnormal tissue remodeling and fibrosis. The involvement of SNAIL in these diseases marks it as a potential target for therapeutic intervention.