Abcam, ab230150, Anti-Clusterin alpha chain antibody [EPR17539-95] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Clusterin antibody. Carrier free. Suitable for IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17539-95,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat,
Applications:IP, IHC-Fr, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab230150 is the carrier-free version of
ab184100
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Clusterin also called apolipoprotein J has an important role in various biological processes. The clusterin alpha chain which is a segment of the clusterin protein is synthesized as part of a precursor that is then cleaved into alpha and beta chains. The mass of this alpha chain is around 34 kDa. Clusterin is expressed in multiple tissues including the liver brain and testes. It often appears in bodily fluids such as plasma and cerebrospinal fluid.
Biological function summary
The clusterin protein operates as a chaperone that prevents non-specific aggregation of proteins which is important in maintaining cellular protein homeostasis. It forms part of a heterodimeric complex composed of alpha and beta chains. This protein also contributes to lipid transport due to its apolipoprotein nature. Its expression levels can increase in response to stress-related conditions suggesting an adaptive function to protect cells.
Pathways
Clusterin is involved in the regulation of cell death and survival pathways. It plays a role in the complement cascade providing protective effects against membrane attack complexes. Clusterin interacts with other proteins such as CRP which assists in modulating these pathways and influences inflammation as part of the immune response.
Disruption of clusterin expression or function relates to neurodegenerative disorders including Alzheimer's disease. The clusterin protein associates with amyloid-beta affecting its aggregation and clearance. It also connects to prostate cancer where altered expression of clusterin might influence tumor cell survival and treatment resistance. Understanding these interactions might offer potential therapeutic strategies with clusterin acting as a target or biomarker in diagnostics or treatment development.