Abcam, ab230263, Anti-TPK1 antibody

Size: 50µL
Rabbit Polyclonal TPK1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TPK1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human TPK1.Q9H3S4

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TPK1 also known as thiamin pyrophosphokinase 1 is an enzyme responsible for the conversion of thiamine (vitamin B1) to thiamine pyrophosphate (TPP). The enzyme facilitates the transfer of pyrophosphate from ATP to thiamine which is the active form needed for cellular metabolism. TPK1 has a molecular mass of approximately 24 kDa. This enzyme is expressed in various tissues including liver kidney and brain reflecting its significant metabolic role.
Biological function summary
TPK1 plays an important role in energy metabolism. Thiamine pyrophosphate the product of TPK1 activity serves as an essential cofactor in several enzyme complexes including pyruvate dehydrogenase complex and alpha-ketoglutarate dehydrogenase complex. These complexes are involved in key metabolic pathways such as glycolysis and the citric acid cycle enabling the cell to produce energy efficiently. Therefore TPK1 activity supports energy production and cellular respiration.
Pathways
TPK1 significantly impacts carbohydrate metabolism and the Krebs cycle. It is involved in the thiamine metabolism pathway which is central to processing carbohydrates and generating energy. Proteins like pyruvate dehydrogenase and transketolase work in conjunction with TPK1 by using the TPP produced. These interactions highlight TPK1's role in linking thiamine conversion and energy-related pathways.
TPK1 mutations can lead to thiamine metabolism dysfunction syndrome type 5 (THMD5) and contribute to Leigh syndrome a severe neurological disorder. TPK1 dysfunction impacts brain metabolism due to its high energy demands. The disorder shares a connection with pyruvate dehydrogenase as both proteins require TPP as a cofactor. Mutations that affect TPK1 can impair these pathways leading to neurological and metabolic complications.