Abcam, ab230499, Anti-MAF1 antibody

Size: 50µL
Rabbit Polyclonal MAF1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAF1 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MAF1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q9H063

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.01% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MAF1 also known as MAF1 homolog is a negative regulator of RNA polymerase III an important enzyme in the synthesis of small RNAs such as tRNA and 5S rRNA. The molecular mass of MAF1 is approximately 29 kDa. This protein mainly expresses in the nucleus of eukaryotic cells. MAF1's activity is controlled by phosphorylation which governs its ability to repress the transcription of RNA polymerase III.
Biological function summary
MAF1 is significant in cellular growth and metabolism regulation. It is not part of a larger protein complex but interacts with several proteins to exert its regulatory functions. MAF1 acts as a brake on RNA polymerase III activity responding to changes in cellular environmental conditions such as nutrient availability and stress. By doing so MAF1 helps coordinate cellular responses to shifts in energy levels and stress modulating the balance between cell growth and maintenance.
Pathways
MAF1 plays a role in the mTOR signaling pathway which is important for cell growth proliferation and survival. Within this pathway MAF1 functions as a downstream effector that inhibits RNA transcription when mTORC1 activity decreases. It relates closely to other proteins such as mTOR and AKT which are also involved in cellular responses to nutrient and energy fluctuations. By influencing RNA polymerase III transcription MAF1 contributes to the regulation of protein synthesis according to metabolic needs.
Alterations in MAF1 activity associate with cancer and metabolic syndromes. In cancer reduced MAF1 activity can lead to unchecked RNA polymerase III activity promoting fast cell proliferation and tumor growth. In metabolic disorders MAF1's involvement in nutrient sensing pathways links to insulin resistance when dysregulated. Through these conditions MAF1 connects to proteins like mTOR and AKT and its malfunction can exacerbate disease progression due to disrupted cell growth control.