Abcam, ab230823, Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23]

Size: 100µL / 1mL
Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823) is a rabbit monoclonal antibody detecting non-muscle Myosin IIB/MYH10 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22564-23,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IP, WB, IHC-P, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] (ab230823) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of non-muscle Myosin IIB/MYH10?
Anti-non-muscle Myosin IIB/MYH10 [EPR22564-23] (ab230823) specifically detects a band for non-muscle Myosin IIB/MYH10 (UniProt: P35580) at a molecular weight of 229kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR22564-23] also available for your convenience: ab230823, Carrier free -
ab254472
ab300647
, Carrier free -
ab300648
, Carrier free -
ab300649
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Non-muscle Myosin IIB also known as MYH10 is a motor protein important in cell division and movement. This protein weighs approximately 228 kDa. Myosin IIB shows expression in various tissues including the brain heart and fibroblasts. Unlike other myosins found in muscle cells Myosin IIB functions mainly in non-muscle cells where it plays a role in organizing intracellular structure and shape.
Biological function summary
The non-muscle Myosin IIB contributes to cell motility and cytokinesis important for normal cell proliferation and maintenance. It operates as part of the actomyosin complex that includes actin and other myosin types. This complex facilitates contractile forces within cells enabling movements such as cell migration and tissue development. Myosin IIB works closely with actin filaments converting chemical energy from ATP into mechanical work required for these processes.
Pathways
Cell migration and tissue morphogenesis involve Myosin IIB. Myosin IIB participates in the Rho-ROCK signaling pathway which regulates actomyosin contraction during cellular movement and morphogenesis. It interacts with other myosins like Myosin IIA illustrating its role in shared pathways. These pathways control the balance between cellular adhesion and detachment necessary for proper cellular function.
Non-muscle Myosin IIB associates with neurological disorders and cancer. Changes in Myosin IIB function can disrupt normal cell division leading to cancer progression. In neurological disorders Myosin IIB affects cell signaling and structural integrity of neurons. For instance disruptions in interactions with actin and other related proteins like Myosin IIA and Tropomyosins can lead to these adverse biological effects showing its significance in maintaining healthy cellular operations.