Abcam, ab231303, Anti-E Cadherin antibody [4A2]

Size: 100µg
Anti-E Cadherin antibody [4A2] (ab231303) is a mouse monoclonal antibody detecting E Cadherin in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Over 140 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4A2,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:The 4A2 monoclonal recognizes the cytoplasmic domain of E-cadherin. The epitope has been mapped to residues 757–778 (PubMed ID: 12393869).

Product details:
Product Specifications
Anti-E Cadherin antibody [4A2] (ab231303) is a mouse monoclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human, mouse, rat samples.
Anti-E Cadherin antibody [4A2] (ab231303) specifically detects E Cadherin (UniProt ID: P12830; Molecular weight: 80kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-E Cadherin antibody [4A2] (ab231303) has high sensitivity and specificity.
The specificity of Anti-E Cadherin antibody [4A2] (ab231303) has been confirmed by testing in knockout samples.
Anti-E Cadherin antibody [4A2] (ab231303) has been cited over 140 times in peer reviewed journals and is trusted by the scientific community.
Related Products
Conjugation-ready, carrier free format available for antibody clone 4A2 -
ab233766
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-Preservative: 0.02% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.
Biological function summary
E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.
Pathways
E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.
E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.