Abcam, ab231692, Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free

Size: 100µg / 1mg
Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (ab231692) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation. Suitable for Western Blot, Flow Cytometry, IHC-P, ICC/IF in Human, Mouse, Rat . - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13030(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-ATP5A antibody [EPR13030(B)] - BSA and Azide free (ab231692) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of ATP5A?
Anti-ATP5A [EPR13030(B)] - BSA and Azide free (ab231692) specifically detects a band for ATP5A (UniProt: P25705) at a molecular weight of 60kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
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ab196198
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ab216385
, Carrier free - ab231692
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP5A also known as ATP synthase F1 subunit alpha is a protein important for cellular energy production. As part of the ATP synthase complex it plays a mechanical role in synthesizing ATP from ADP and inorganic phosphate. The ATP5A protein has a molecular weight of approximately 55 kDa and is widely expressed in the inner mitochondrial membrane across different cell types. Its central function lies in its ability to harness the energy of the proton gradient generated by the electron transport chain to catalyze ATP synthesis.
Biological function summary
ATP5A is essential in cellular respiration serving as a catalytic core of the F1 component of ATP synthase. As part of the multi-subunit enzyme complex ATP synthase is responsible for ATP production the primary energy currency in cells. The ATP5A subunit works in conjunction with other subunits of the enzyme oligomer to facilitate the conversion of energy released during oxidative phosphorylation into a usable form. The protein's efficiency in this biological role underpins its importance in sustaining cellular energy homeostasis.
Pathways
ATP5A plays a pivotal role in oxidative phosphorylation and the electron transport chain integral components of cellular respiration. The oxidative phosphorylation pathway depends on this protein to manage the synthesis of ATP molecules while the electron transport chain creates the proton gradient necessary for ATP production. ATP5A is functionally connected to other proteins in these pathways such as ATP5B and cytochrome c oxidase working in a coordinated manner to ensure efficient energy transfer and maintenance.
ATP5A is implicative in mitochondrial disorders and neurodegenerative diseases such as Leigh syndrome and Parkinson's disease. These conditions often arise from deficits in ATP production where ineffective ATP synthase activity can contribute to cellular energy failures. In the context of Parkinson's disease for instance ATP5A interactions with other proteins like Parkin can contribute to mitochondrial dysfunction an important pathological feature of the disorder. Through such associations alterations in ATP5A activity can significantly impact disease progression and symptomatology.