Abcam, ab232551, Anti-Serine/threonine-protein kinase 4/MST-1 antibody [EP1465Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Serine/threonine-protein kinase 4/MST-1 antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1465Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IP, WB, IHC-P, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab232551 is the carrier-free version of
ab51134
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Serine/threonine-protein kinase 4 (MST-1) also known as STK4 is a 52 kDa enzyme that plays an important role in cellular signaling processes. This kinase ubiquitously expresses in many tissues including liver kidney and heart. MST-1 functions as a part of a complex facilitating its catalytic activity by activating downstream substrates. The alternative name for this protein is mammalian STE20-like protein kinase 1 linking MST-1 to a family of serine/threonine kinases.
Biological function summary
This kinase orchestrates various cellular processes mainly through regulation of apoptosis and cell stress responses. MST-1 interacts with other signaling molecules in cells forming part of larger multi-protein complexes. These interactions allow MST-1 to act as a molecular switch effectively controlling the balance between cell survival and death. The cellular localization and interaction partners help diversify its biological roles.
Pathways
MST-1 predominantly influences the Hippo signaling pathway—a pathway significant in controlling organ size and suppressing cancer. In this pathway MST-1 phosphorylates and activates LATS1/2 kinases creating an endpoint effect on cell proliferation and apoptosis. MST-1 also participates in the oxidative stress response pathway where it shows interaction with FoxO transcription factors leading to modifications in gene expression profiles.
The dysregulation of MST-1 is linked to cancer and cardiovascular diseases. In cancer the Hippo pathway's consistent activation by MST-1 helps modulate uncontrolled cell division while in cardiovascular diseases MST-1 plays a role by driving cardiomyocyte apoptosis. MST-1’s interplay with FoxO transcription factors also highlights its contribution to these diseases by reinforcing stress response and DNA repair mechanisms.