Abcam, ab232558, Anti-Myogenin antibody [EPR4789] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Myogenin antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4789,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody has low affinity on skeletal muscle samples in WB. Please increase protein or antibody amount to get satisfied results.

Product details:
ab232558 is the carrier-free version of
ab124800
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Myogenin also known as MYOG is a muscle-specific transcription factor that plays an important role in the regulation of muscle differentiation and development. It belongs to the MyoD family of basic helix-loop-helix proteins. Myogenin has a molecular weight of approximately 34 to 39 kDa. It is typically expressed in skeletal muscle tissues aiding in the transition from proliferating myoblasts to differentiated myotubes. Immunohistochemistry (IHC) methods often use myogenin antibodies like the 'myogenin anticuerpo' and 'F5D' to identify myogenin expression within tissue samples facilitating the understanding of muscle tissue organization and development.
Biological function summary
Myogenin functions in controlling the transcription of muscle-specific genes that are essential during myogenesis. This transcription factor forms heterodimers with E-proteins becoming part of DNA-binding complexes that activate the transcription of muscle-specific genes. Myogenin's presence is critical during muscle cell fusion and maturation signifying its importance in proper muscle tissue formation and maintenance. Myogenin staining is often used as a technique to study muscle differentiation in various experimental settings.
Pathways
Myogenin is integrated into the myogenic regulatory network where it activates genes involved in muscle fiber maturation. It participates in the MyoD regulatory pathway working alongside other myogenic regulatory factors like MyoD Myf5 and MRF4. These proteins create a coordinated network ensuring efficient muscle cell differentiation and growth. The cross-talk between myogenin and these factors highlights its role in the intricate web of gene regulation necessary for muscle development.
The altered expression or mutation of the myogenin gene has potential implications in muscular dystrophies and myopathies. For instance disruptions in myogenin activity can lead to impaired muscle repair and regeneration contributing to the pathology of conditions such as rhabdomyosarcoma. Within these contexts myogenin associates with other proteins like MyoD whose expression might also be altered further accentuating the disruption in normal muscle differentiation processes during disease states.