Abcam, ab232822, Anti-NAT2/AT-2 antibody

Size: 100µg
Rabbit Polyclonal NAT2/AT-2 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Rat Nat2 aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Rat Nat2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P50298

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antigen-specific affinity chromatography followed by Protein A affinity chromatography., Storage buffer-pH: 7.4Preservative: 0.011% Proclin 300Constituents: PBS, 55.77% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NAT2 also known as N-acetyltransferase 2 or AT-2 is an important enzyme in drug metabolism. It catalyzes the acetylation process which plays a role in converting substances such as drugs and environmental chemicals into less active forms. This enzymatic activity requires co-factor acetyl-CoA. NAT2 has a molecular weight of about 33 kDa and is expressed mostly in liver and intestines. Its genetic polymorphisms influence enzyme activity leading to rapid or slow acetylator phenotypes among individuals.
Biological function summary
NAT2 participates in the metabolism of aromatic amines which includes the acetylation of drugs like isoniazid and sulfamethazine. NAT2 does not typically form complexes with other proteins but its activity affects the concentration of metabolites that can interact with other pathways or proteins. It regulates the bioavailability and potential toxicity of different chemical substances in the body by modifying their structure and reactivity impacting pharmacokinetics significantly.
Pathways
NAT2 is deeply involved in xenobiotic metabolism and Phase II drug metabolism pathways. It contributes directly to the N-acetylation pathway critical for the biotransformation of pharmaceuticals. NAT2 works in tandem with Cytochrome P450 enzymes which usually perform initial oxidative modifications on compounds destined for NAT2 acetylation. This interaction highlights NAT2's role in creating metabolic balance by processing potential toxins and facilitating their removal or detoxification from the body.
NAT2 polymorphisms play a critical role in susceptibility to certain conditions such as bladder cancer and drug-induced lupus erythematosus. Slow acetylator phenotypes are associated with higher risk of bladder cancer due to inefficient detoxification of carcinogens. Furthermore NAT2 interplays with proteins like N-acetyltransferase 1 (NAT1) influencing the biological responses and effectiveness of therapeutic strategies. Understanding the role of NAT2 in these disorders is essential for developing personalized medicine approaches to treatment.