Abcam, ab232964, Anti-HIF1 beta antibody

Size: 100µg
Rabbit Polyclonal HIF1 beta antibody. Suitable for WB, ICC/IF and reacts with Rat, Pig, Recombinant fragment - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARNT aa 600 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human, Pig,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ARNT aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P27540

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab232964 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 55.77% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIF1 beta also known as ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) plays an important role in cellular response to low oxygen levels. It partners with HIF1 alpha to form the hypoxia-inducible factor (HIF) complex. This beta subunit has a molecular weight of approximately 90 kDa and expresses ubiquitously in many cell types across different tissues. As part of the HIF complex HIF1 beta acts as a critical transcriptional regulator during hypoxic conditions.
Biological function summary
HIF1 beta regulates the expression of genes involved in adaptation to hypoxia. It functions as a dimerization partner in the HIF complex joining with HIF1 alpha or other proteins in the PAS superfamily to activate transcription of target genes. These genes are important for processes like angiogenesis metastasis and energy metabolism helping cells adapt to oxygen-deprived environments. The protein's interaction mainly influences cellular responses to hypoxia supporting tissue survival and function under stress.
Pathways
The involvement of HIF1 beta in the cellular hypoxia response is significant. It engages in the HIF pathway interacting closely with HIF1 alpha promoting the transcription of hypoxia-responsive genes when oxygen levels drop. This process integrates with larger pathways like the VEGF signaling pathway vital for blood vessel formation. Moreover its connection to ARNT serves motivation in the regulation of circadian rhythms showing the protein's broad relevance in cellular regulation.
HIF1 beta has associations with cancer and ischemic conditions. Aberrant activation of the HIF pathway where HIF1 beta is an important component can drive tumor progression through increased angiogenesis and metabolic reprogramming connecting it indirectly to VEGF. In ischemia altered gene expression mediated by HIF1 beta can influence tissue response to low oxygen contributing to disease pathology. Therefore targeting HIF1 beta might offer potential therapeutic avenues for these conditions.