Abcam, ab233727, Anti-Argonaute-2 antibody [EPR10411] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Argonaute-2 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 13 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10411,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:Flow Cyt (Intra), ICC/IF, IP, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab233727 is the carrier-free version of
ab186733
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Argonaute-2 often referred to as Ago2 or Argonaute-2 protein plays an important role in the RNA-induced silencing complex (RISC). It has a molecular weight of approximately 97 kDa. Ago2 is found in the cytoplasm and nucleus of cells and is expressed in a wide range of tissues including the brain and liver. Its mechanical function involves binding to guide RNA and facilitating strand cleavage which is essential for gene silencing and regulation at the post-transcriptional level.
Biological function summary
The Argonaute-2 protein participates in gene silencing mechanisms. As a part of RISC Ago2 binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) to direct sequence-specific cleavage of target mRNAs regulating their translation and stability. This protein is a part of a multi-protein complex that modulates gene expression and influences cellular homeostasis and development. In its biological role Argonaute-2 impacts cellular differentiation proliferation and apoptosis.
Pathways
Several cellular processes require Argonaute-2 notably the RNA interference (RNAi) and microRNA (miRNA) pathways. In these pathways it interacts with Dicer a protein essential for the maturation of small RNA molecules and facilitates the gene silencing process. Ago2 is also involved in the regulation of gene expression during embryonic development and stress responses indicating its importance in maintaining cellular function and responding to environmental changes.
Anomalies in Argonaute-2 activity correlate with cancer including lung and breast cancer where dysregulation of miRNA pathways can lead to unchecked cellular proliferation. It is also linked to neurological disorders like schizophrenia possibly due to its role in gene expression regulation in neural cells. Interactions between Ago2 and proteins like Dicer and other Argonautes suggest complex regulatory networks that when disrupted contribute to these diseases.