Abcam, ab233753, Anti-APC2 antibody [3A2G2]

Size: 100µg
Mouse Monoclonal APC2 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human APC2 aa 2000-2200.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3A2G2,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human APC2 aa 2000-2200. The exact immunogen used to generate this antibody is proprietary information.O95996

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APC2 also known as Adenomatous Polyposis Coli 2 is a critical regulator of cell function primarily involved in the cell cycle and stability of beta-catenin. This protein has a molecular mass of approximately 260 kDa and is mainly expressed in neural tissue and embryonic development stages. APC2 shares structural similarities with APC1 including the presence of armadillo repeats which facilitate protein-protein interactions necessary for its function.
Biological function summary
APC2 interacts with cytoskeletal networks and contributes to cellular architecture and mobility. It is a component of the WNT signaling cascade mainly directing the degradation of beta-catenin which prevents irregular cell proliferation. APC2 forms part of the destruction complex working alongside proteins like Axin and GSK3β to regulate beta-catenin levels within cells.
Pathways
APC2 plays a significant role in the WNT signaling pathway important for cellular growth and communication. Through this pathway APC2 interacts with various proteins such as beta-catenin and GSK3β controlling their activity and stability. Its function in this pathway impacts gene expression which is critical for processes like embryonic development and adult tissue homeostasis.
Mutations or dysregulation of APC2 have been associated with colorectal cancer and neural developmental anomalies. These conditions often involve aberrations in the WNT signaling pathway where APC2 fails to regulate beta-catenin adequately. Research has linked APC2 with APC1 where both proteins contribute to oncogenic processes when their regulatory roles in beta-catenin degradation are compromised.